Development and Study of Antibacterial <i>In situ</i> Coatings for Processing Implants

Introduction. Periprosthetic infections are a major problem in orthopedic surgery. The most optimal way to influence the bacterial film is suppress early stages of its formation. use hydrogel coatings for prevention periprosthetic an effective measure, while process coating implant can be simplified due situ systems. Aim. purpose research develop and study antibacterial preoperative treatment implants. Materials methods. In experiment we used poloxamers Kolliphor® P 407 188 (BASF, Germany), hyaluronic acid high-molecular PrincipHYAL® (1400–1800 kDa), low-molecular (400–600 mixture high, medium acids Cube3 (ROELMI HPC Srl, Italy). As screening methods identifying composition, gelation temperature measurements, pH studies rheological properties, microbiological tests. Results discussion. During experiment, found concentration 1400–1800 kDa – 0.5 %, at which studied composition 4.5 ± °C was homogeneous liquid, it made sol-gel transition when heated. It revealed that had no significant effect on gelling temperature, so added poloxamer 188. We also established molecular weight systems characteristics. High stabilized viscosity values improved adhesive properties system, samples with low showed lower dynamic phase gel-sol end. tested (containing 18.0 % 407, 2 188, kDa) adhesion titanium plate. When exposed paddle stirrer (rotation speed 20 rpm) 15 minutes stored thermostat (37,0 0,5 °C) week, analyzed strength, makes possible coating. Conclusion. By multistage sample selected introduction model component representing cocktail bacteriophages Klebsiella pneumonia , Staphylococcus aureus Escherichia coli . Microbiological good compatibility immunobiological substance excipients, indicated prospects further preclinical studies.